Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP1-84854 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-84854, RRID:AB_11037833
- Product name
- Rabbit Polyclonal ETFA Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, mouse, rat ETFA antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: ETFA Antibody [NBP1-84854] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: ETFA Antibody [NBP1-84854] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: ETFA Antibody [NBP1-84854] - Analysis in U2OS cells transfected with control siRNA, target specific siRNA probe #1 and #2, using anti-ETFA antibody. Remaining relative intensity is presented.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84854] - Staining of human rectum shows strong cytoplasmic positivity in glandular cells.
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84854] - Staining of human colon shows strong granular cytoplasmic positivity in glandular cells.
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84854] - Staining of human kidney shows strong granular cytoplasmic positivity in cells in tubules.
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84854] - Staining of human liver shows strong granular cytoplasmic positivity in hepatocytes.
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84854] - Staining of human pancreas shows moderate granular cytoplasmic positivity in exocrine glandular cells.
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ETFA Antibody [NBP1-84854] - Staining of human colon, kidney, liver and pancreas using Anti-ETFA antibody NBP1-84854 (A) shows similar protein distribution across tissues to independent antibody NBP1-84856 (B).