Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
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Validation data
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- Product number
- PA5-28201 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ETFA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, PC-12.
- Concentration
- 0.88 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ETFA was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ETFA Polyclonal Antibody (Product # PA5-28201) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using ETFA Polyclonal Antibody (Product # PA5-28201). Sample (30 µg of whole cell lysate). Lane A: A431. Lane B: HeLa. 10% SDS PAGE. ETFA Polyclonal Antibody (Product # PA5-28201) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ETFA Polyclonal Antibody detects ETFA protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract.10 % SDS-PAGE. ETFA Polyclonal Antibody (Product # PA5-28201) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ETFA in methanol-fixed HeLa cells using an ETFA polyclonal antibody (Product # PA5-28201) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ETFA Polyclonal Antibody detects ETFA protein at mitochondria on mouse heart by immunohistochemical analysis. Sample: Paraffin-embedded mouse stomach. ETFA Polyclonal Antibody (Product # PA5-28201) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ETFA Polyclonal Antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse heart. ETFA Polyclonal Antibody (Product # PA5-28201) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ETFA Polyclonal Antibody detects ETFA protein at mitochondria on mouse liver by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ETFA Polyclonal Antibody (Product # PA5-28201) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using ETFA (Product # PA5-28201) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.