PA5-30021

antibody from Invitrogen Antibodies

Targeting: ETFA EMA, GA2, MADD

Immunoprecipitation

Antibody data

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Validation data

Product number: PA5-30021 - Provider product page
Provider: Invitrogen Antibodies
Product name: ETFA Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Recombinant protein fragment
Description: Recommended positive controls: 293T, A431, HeLa, HepG2, C8D30, PC-12. Predicted reactivity: Mouse (96%), Rat (95%), Zebrafish (84%), Xenopus laevis (85%), Cat (96%), Pig (96%), Chicken (87%), Rhesus Monkey (98%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Isotype: IgG
Vial size: 100 µL
Concentration: 0.91 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ETFA protein structure - PA5-30021 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of ETFA using Various whole cell extracts (30 µg). Samples were loaded onto a 12% SDS-PAGE gel and probed with an ETFA polyclonal antibody (Product # PA5-30021) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of ETFA was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ETFA Polyclonal Antibody (Product # PA5-30021) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: ETFA Polyclonal Antibody detects ETFA protein by Western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA Polyclonal Antibody (Product # PA5-30021) diluted by 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: ETFA Polyclonal Antibody detects ETFA protein by Western blot analysis. A. 30 µg C8D30 whole cell lysate/extract.10 % SDS-PAGE. ETFA Polyclonal Antibody (Product # PA5-30021) dilution: 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: ETFA Polyclonal Antibody detects ETFA protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract.10 % SDS-PAGE. ETFA Polyclonal Antibody (Product # PA5-30021) dilution: 1:1,000.