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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunocytochemistry [1]
- Immunohistochemistry [4]
- Other assay [1]
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- Product number
- PA5-30021 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ETFA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, C8D30, PC-12.
- Concentration
- 0.91 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ETFA using Various whole cell extracts (30 µg). Samples were loaded onto a 12% SDS-PAGE gel and probed with an ETFA polyclonal antibody (Product # PA5-30021) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of ETFA was performed by separating 30 µg of non-transfected (–) and transfected (+) 293T whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a ETFA Polyclonal Antibody (Product # PA5-30021) at a dilution of 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ETFA Polyclonal Antibody detects ETFA protein by Western blot analysis. Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with ETFA Polyclonal Antibody (Product # PA5-30021) diluted by 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ETFA Polyclonal Antibody detects ETFA protein by Western blot analysis. A. 30 µg C8D30 whole cell lysate/extract.10 % SDS-PAGE. ETFA Polyclonal Antibody (Product # PA5-30021) dilution: 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ETFA Polyclonal Antibody detects ETFA protein by Western blot analysis. A. 30 µg PC-12 whole cell lysate/extract.10 % SDS-PAGE. ETFA Polyclonal Antibody (Product # PA5-30021) dilution: 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ETFA in methanol-fixed HeLa cells using an ETFA polyclonal antibody (Product # PA5-30021) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ETFA Polyclonal Antibody detects ETFA protein at mitochondria on mouse brain stem by immunohistochemical analysis. Sample: Paraffin-embedded mouse kidney. ETFA Polyclonal Antibody (Product # PA5-30021) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ETFA Polyclonal Antibody detects ETFA protein at mitochondria on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. ETFA Polyclonal Antibody (Product # PA5-30021) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ETFA Polyclonal Antibody detects ETFA protein at mitochondria on mouse kidney by immunohistochemical analysis. Sample: Paraffin-embedded mouse liver. ETFA Polyclonal Antibody (Product # PA5-30021) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ETFA Polyclonal Antibody detects ETFA protein at mitochondria on mouse urinary bladder by immunohistochemical analysis. Sample: Paraffin-embedded mouse urinary bladder. ETFA Polyclonal Antibody (Product # PA5-30021) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of ETFA was performed in HepG2 whole cell extracts using 5 µg of ETFA Polyclonal Antibody (Product # PA5-30021). Samples were transferred to a membrane and probed with ETFA Polyclonal Antibody as a primary antibody and an HRP-conjugated anti-Rabbit IgG was used as a secondary antibody.
