Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-53439 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ABCG5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: IHQPRSELFQ LFDKIAILSF GELIFCGTPA EMLDFFNDCG YPCPEHSNPF DFYMDLTSVD TQSKEREIET SKRVQMIESA YKKSAICHKT LKNIERMKHL KTLPMVPFKT KDSPGVFSKL GVLL Highest antigen sequence identity to the following orthologs: Mouse - 81%, Rat - 81%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.3 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Caco-2 Cells for Measuring Intestinal Cholesterol Transport - Possibilities and Limitations.
Hiebl V, Schachner D, Ladurner A, Heiss EH, Stangl H, Dirsch VM
Biological procedures online 2020;22:7
Biological procedures online 2020;22:7
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of ABCG5 in human cell line Hep G2 using an ABCG5 Polyclonal Antibody (Product # PA5-53439) shows localization to nucleus.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 6 a-c Confocal laser scanning microscopy for the detection of ABCG5, NPC1L1 and ABCB1. Staining was carried out using Protocol #6. Cell nuclei are shown in blue (DAPI) and ABCG5, NPC1L1 or ABCB1 in green (fluorescently labelled antibody). Representative images of ABCG5 ( a ), treated with the vehicle control (DMSO 0.1%, left image) or GW3965 (LXR agonist, 10 muM, middle image). Representative images of NPC1L1 ( b ), treated with the vehicle control (DMSO 0.1%, left image) or GW3965 (LXR agonist, 10 muM, middle image). Representative images of ABCB1 ( c ), treated with the vehicle control (DMSO 0.1%, left image) or bexarotene (RXR agonist, 5 muM, middle image). The image on the right ( a-c ) is the respective control only stained with DAPI and the secondary antibody, showing the unspecific binding of the secondary antibody. Shown are maximum projections of the top views