Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AP33457PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Product name
- anti GFRA3 / GDNFR-alpha 3
- Antibody type
- Polyclonal
- Antigen
- Recombinant His-tagged human GFRa-3 protein produced using CHO-based. For production of hGFRa-3, glycosylphosphatidyl-inositol GPI-anchor was removed and protein was secreted to the cell culture supernatant. Protein was purified by Ni-affinity chromatography following gel-filtration from cell culture supernatant.
- Reactivity
- Human
- Host
- Chicken/Avian
- Isotype
- IgY
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western-Blot detection of human GFRα-3, using Chicken polyclonal antibody to human GFRα-3. GFRα-3 was C-terminally His tagged. Protein was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Lane 1. Prestained protein ladder (Naxo 8003). Lane 2. hGFRα-3. Primary antibody dilution 1:5000 was used. Rabbit anti-chicken antibody was used as secondary antibody.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- IF detection of human GFRα-3 expressed in U2OS cells. GFRα-3 was visualized using Chicken polyclonal antibody to human GFRα-3 (dilution 1:3000) and Goat anti-chicken IgY-H&L DyLight 550 (dilution 1:200) as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for GFRα-3 specific signal and blue for nuclei.