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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- AP33457PU-N - Provider product page
- Provider
- OriGene
- Product name
- GFR alpha 3 (GFRA3) chicken polyclonal antibody, Aff - Purified
- Antibody type
- Polyclonal
- Description
- GFR alpha 3 (GFRA3) chicken polyclonal antibody, Aff - Purified
- Host
- Chicken/Avian
- Conjugate
- Unconjugated
- Epitope
- GFRA3
- Isotype
- IgY
- Antibody clone number
- NULL
- Vial size
- 100 µg
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western-Blot detection of human GFRa-3, using Chicken polyclonal antibody to human GFRa-3. GFRa-3 was C-terminally His tagged. Protein was expressed by CHO cell culture. 100 ng of purified protein was loaded per lane. Lane 1. Prestained protein ladder (Naxo 8003). Lane 2. hGFRa-3. Primary antibody dilution 1:5000 was used. Rabbit anti-chicken antibody was used as secondary antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- IF detection of human GFRa-3 expressed in U2OS cells. GFRa-3 was visualized using Chicken polyclonal antibody to human GFRa-3 (dilution 1:3000) and Goat anti-chicken IgY-H&L DyLight 550 (dilution 1:200) as secondary antibody. For nuclear staining DAPI was used. ArrayScan VTI platform (Thermo Scientific) was used for image acquisition (10x objective). Composite picture was generated using pseudocolors green for GFRa-3 specific signal and blue for nuclei.
- Validation comment
- IF
