AF670

antibody from R&D Systems

Targeting: GFRA3 GFRa-3

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Antibody Data

Product number

AF670

Provider

R&D Systems

Product name

Human GFR alpha-3/GDNF R alpha-3 Antibody

Antibody type

Polyclonal

Description

Antigen Affinity-purified. Detects human GFR alpha-3/GDNF R alpha-3 in direct ELISAs and Western blots. In direct ELISA and Western blots, no cross-reactivity with recombinant human GFR alpha-2 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

AAC24355


Isotype

IgG

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

Functional mapping of receptor tyrosine kinases in myxoid liposarcoma.

Negri T, Virdis E, Brich S, Bozzi F, Tamborini E, Tarantino E, Jocollè G, Cassinelli G, Grosso F, Sanfilippo R, Casalini P, Greco A, Pierotti MA, Pilotti S
Clinical cancer research : an official journal of the American Association for Cancer Research 2010 Jul 15;16(14):3581-93

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human GFR alpha-3/GDNF R alpha-3 by Western Blot. Western blot shows lysates of human stomach tissue and human small intestine tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human GFR alpha-3/GDNF R alpha-3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF670) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). Specific bands were detected for GFR alpha-3/GDNF R alpha-3 at approximately 55 and 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.