Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [6]
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Validation data
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- Product number
- PA5-54513 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Actin-like 7B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: MATRNSPMPL GTAQGDPGEA GTRPGPDASL RDTGAATQLK MKPRKVHKIK AVIIDLGSQY CKCGYAGEPR PTYFISSTVG KRCPEAADAG DTRKWTL Highest antigen sequence identity to the following orthologs: Mouse - 74%, Rat - 76%.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Actin-like 7B in control (vector only transfected HEK293T lysate) and ACTL7B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a Actin-like 7B Polyclonal Antibody (Product # PA5-54513).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Actin-like 7B in control (vector only transfected HEK293T lysate) and ACTL7B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells). Samples were probed using a Actin-like 7B Polyclonal Antibody (Product # PA5-54513).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Actin-like 7B in human testis and kidney tissues using Actin-like 7B Polyclonal Antibody (Product # PA5-54513). Corresponding ACTL7B RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Actin-like 7B in human cerebral cortex, duodenum, kidney and testis using Actin-like 7B Polyclonal Antibody (Product # PA5-54513) (A) shows similar protein distribution across tissues to an independent Actin-like 7B Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Actin-like 7B in human testis using Actin-like 7B Polyclonal Antibody (Product # PA5-54513) shows strong nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Actin-like 7B in human kidney using Actin-like 7B Polyclonal Antibody (Product # PA5-54513) shows no positivity in cells in tubules as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Actin-like 7B in human duodenum using Actin-like 7B Polyclonal Antibody (Product # PA5-54513) shows no positivity in glandular cells as expected.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Actin-like 7B in human cerebral cortex using Actin-like 7B Polyclonal Antibody (Product # PA5-54513) shows no positivity in neurons as expected.