Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [6]
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Validation data
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- Product number
- NBP1-86973 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-86973, RRID:AB_11008855
- Product name
- Rabbit Polyclonal ACTL7B Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human ACTL7B antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Mouse Tafazzin Is Required for Male Germ Cell Meiosis and Spermatogenesis.
The human testis-specific proteome defined by transcriptomics and antibody-based profiling.
Antibodies biotinylated using a synthetic Z-domain from protein A provide stringent in situ protein detection.
Cadalbert LC, Ghaffar FN, Stevenson D, Bryson S, Vaz FM, Gottlieb E, Strathdee D
PloS one 2015;10(6):e0131066
PloS one 2015;10(6):e0131066
The human testis-specific proteome defined by transcriptomics and antibody-based profiling.
Djureinovic D, Fagerberg L, Hallström B, Danielsson A, Lindskog C, Uhlén M, Pontén F
Molecular human reproduction 2014 Jun;20(6):476-88
Molecular human reproduction 2014 Jun;20(6):476-88
Antibodies biotinylated using a synthetic Z-domain from protein A provide stringent in situ protein detection.
Andersson S, Konrad A, Ashok N, Pontén F, Hober S, Asplund A
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2013 Nov;61(11):773-84
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society 2013 Nov;61(11):773-84
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ACTL7B Antibody [NBP1-86973] - Analysis in control (vector only transfected HEK293T lysate) and ACTL7B over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACTL7B Antibody [NBP1-86973] - Analysis in human testis and kidney tissues. Corresponding ACTL7B RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACTL7B Antibody [NBP1-86973] - Staining of human kidney shows no positivity in cells in tubules as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACTL7B Antibody [NBP1-86973] - Staining of human duodenum shows no positivity in glandular cells as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACTL7B Antibody [NBP1-86973] - Staining of human cerebral cortex shows no positivity in neurons as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACTL7B Antibody [NBP1-86973] - Staining of human testis shows strong nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ACTL7B Antibody [NBP1-86973] - Staining of human cerebral cortex, duodenum, kidney and testis using Anti-ACTL7B antibody NBP1-86973 (A) shows similar protein distribution across tissues to independent antibody NBP1-86972 (B).