H00007408-D01P

antibody from Abnova Corporation

Targeting: VASP

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00007408-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00007408-D01P, RRID:AB_1581839

Product name

VASP purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human VASP protein.

Antigen sequence

MSETVICSSRATVMLYDDGNKRWLPAGTGPQAFSR
VQIYHNPTANSFRVVGRKMQPDQQVVINCAIVRGV
KYNQATPNFHQWRDARQVWGLNFGSKEDAAQFAAG
MASALEALEGGGPPPPPALPTWSVPNGPSPEEVEQ
QKRQQPGPSEHIERRVSNAGGPPAPPAGGPPPPPG
PPPPPGPPPPPGLPPSGVPAAAHGAGGGPPPAPPL
PAAQGPGGGGAGAPGLAAAIAGAKLRKVSKQEEAS
GGPTAPKAESGRSGGGGLMEEMNAMLARRRKATQV
GEKTPKDESANQEEPEARVPAQSESVRRPWEKNST
TLPRMKSSSSVTTSETQPCTPSSSDYSDLQRVKQE
LLEEVKKELQKVKEEIIEAFVQELRKRGSP


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of VASP expression in transfected 293T cell line (H00007408-T01) by VASP MaxPab polyclonal antibody.Lane 1: VASP transfected lysate(39.80 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between VASP and ACTG1. HeLa cells were stained with anti-VASP rabbit purified polyclonal 1:1200 and anti-ACTG1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)