Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- 615602 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#615602, RRID:AB_2213417
- Product name
- Purified anti-VASP
- Antibody type
- Polyclonal
- Antigen
- Recombinant (partial), C-terminal
- Description
- VASP (vasodilator-stimulated phosphoprotein) is a 46 kD member of the Ena/VASP family that contains WH1, EVH1, and EVH2 domains as well as GPPPPP motifs. This protein forms tetramers and may act in concert with profilin to convey signal transduction to actin filament production. VASP is a crucial factor involved in enhancement of actin filament formation and has been shown to regulatee cell motility by controlling the geometry of actin filament networks within lamellipodia. VASP can be phosphorylated by PKA and PKG; phosphorylation disrupts protein associations. VASP has been shown to interact with PKA, PKG, actin, vinculin, zyxin, profilin, F-actin, and c-abl. The Poly6156 antibody recognizes the C-terminal region of human and mouse VASP and has been shown to be useful for Western blotting.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 200 µl
- Storage
- Upon receipt, store frozen at -20° C.
Submitted references Cyclic GMP kinase and RhoA Ser188 phosphorylation integrate pro- and antifibrotic signals in blood vessels.
Sawada N, Itoh H, Miyashita K, Tsujimoto H, Sone M, Yamahara K, Arany ZP, Hofmann F, Nakao K
Molecular and cellular biology 2009 Nov;29(22):6018-32
Molecular and cellular biology 2009 Nov;29(22):6018-32
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- HepG2 cell extract was resolved by electrophoresis, transferred to nitrocellulose, and probed with rabbit anti-VASP antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system.
- Conjugate
- Unconjugated