HPA005724

antibody from Atlas Antibodies

Targeting: VASP

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

HPA005724

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA005724, RRID:AB_1858721

Product name

Anti-VASP

Antibody type

Polyclonal

Description

Polyclonal Antibody against Human VASP, Gene description: vasodilator-stimulated phosphoprotein, Validated applications: WB, IHC, ICC, Uniprot ID: P50552, Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Isotype

IgG

Vial size

100 µl

Concentration

0.1 mg/ml

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Handling

The antibody solution should be gently mixed before use.

References

Submitted references

NHSL3 controls single and collective cell migration through two distinct mechanisms

Novikov N, Gao J, Fokin A, Rocques N, Chiappetta G, Rysenkova K, Zea D, Polesskaya A, Vinh J, Guerois R, Gautreau A
Nature Communications 2025;16(1)

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ArpC5 isoforms regulate Arp2/3 complex–dependent protrusion through differential Ena/VASP positioning

Fäßler F, Javoor M, Datler J, Döring H, Hofer F, Dimchev G, Hodirnau V, Faix J, Rottner K, Schur F
Science Advances 2023;9(3)

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Targeting CaMKK2 Inhibits Actin Cytoskeletal Assembly to Suppress Cancer Metastasis

Mukherjee D, Previs R, Haines C, Al Abo M, Juras P, Strickland K, Chakraborty B, Artham S, Whitaker R, Hebert K, Fontenot J, Patierno S, Freedman J, Lau F, Burow M, Chang C, McDonnell D
Cancer Research 2023;83(17):2889-2907

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Actin-rich lamellipodia-like protrusions contribute to the integrity of epithelial cell–cell junctions

Senju Y, Mushtaq T, Vihinen H, Manninen A, Saarikangas J, Ven K, Engel U, Varjosalo M, Jokitalo E, Lappalainen P
Journal of Biological Chemistry 2023;299(5):104571

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Ena/VASP Protein-Mediated Actin Polymerization Contributes to Naïve CD8+ T Cell Activation and Expansion by Promoting T Cell–APC Interactions In Vivo

Waldman M, Rahkola J, Sigler A, Chung J, Willett B, Kedl R, Friedman R, Jacobelli J
Frontiers in Immunology 2022;13

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Generation of contractile actomyosin bundles depends on mechanosensitive actin filament assembly and disassembly

Tojkander S, Gateva G, Husain A, Krishnan R, Lappalainen P
eLife 2015;4

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Systematic validation of antibody binding and protein subcellular localization using siRNA and confocal microscopy

Stadler C, Hjelmare M, Neumann B, Jonasson K, Pepperkok R, Uhlén M, Lundberg E
Journal of Proteomics 2012;75(7):2236-2251

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane, cytosol & focal adhesion sites.

Sample type

Human

Immunohistochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human lymph node and skeletal muscle tissues using HPA005724 antibody. Corresponding VASP RNA-seq data are presented for the same tissues.

Sample type

Human

Protocol

Protocol

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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