GTX110274

antibody from GeneTex

Targeting: ELP1 DYS, IKAP, IKBKAP, IKI3, TOT1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX110274

Provider

GeneTex

Proper citation

GeneTex Cat#GTX110274, RRID:AB_10724877

Product name

IKAP antibody [C1C2], Internal

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

Capturing the biology of disease severity in a PSC-based model of familial dysautonomia.

Zeltner N, Fattahi F, Dubois NC, Saurat N, Lafaille F, Shang L, Zimmer B, Tchieu J, Soliman MA, Lee G, Casanova JL, Studer L
Nature medicine 2016 Dec;22(12):1421-1427

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: HeLa B: HepG2 5% SDS PAGE GTX110274 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IKAP antibody [C1C2], Internal detects IKAP protein by western blot analysis.A. 30 ?g GL261 whole cell lysate/extract B. 30 ?g Raw264.7 whole cell lysate/extract5% SDS-PAGEIKAP antibody [C1C2], Internal (GTX110274) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

IKAP antibody [C1C2], Internal detects IKAP protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: IKAP protein stained by IKBKAP antibody [C1C2], Internal (GTX110274) diluted at 1:500.Blue: Hoechst 33343 staining.