Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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Validation data
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- Product number
- GTX110274 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX110274, RRID:AB_10724877
- Product name
- IKAP antibody [C1C2], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Capturing the biology of disease severity in a PSC-based model of familial dysautonomia.
Zeltner N, Fattahi F, Dubois NC, Saurat N, Lafaille F, Shang L, Zimmer B, Tchieu J, Soliman MA, Lee G, Casanova JL, Studer L
Nature medicine 2016 Dec;22(12):1421-1427
Nature medicine 2016 Dec;22(12):1421-1427
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: HeLa B: HepG2 5% SDS PAGE GTX110274 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IKAP antibody [C1C2], Internal detects IKAP protein by western blot analysis.A. 30 ?g GL261 whole cell lysate/extract B. 30 ?g Raw264.7 whole cell lysate/extract5% SDS-PAGEIKAP antibody [C1C2], Internal (GTX110274) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IKAP antibody [C1C2], Internal detects IKAP protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: IKAP protein stained by IKBKAP antibody [C1C2], Internal (GTX110274) diluted at 1:500.Blue: Hoechst 33343 staining.