PA5-87654

antibody from Invitrogen Antibodies

Targeting: ELP1 DYS, IKAP, IKBKAP, IKI3, TOT1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-87654

Provider

Invitrogen Antibodies

Product name

IKAP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: LRLVYKYNRL DIIETNVKPS ILEAQKNYMA FLDSQTATFS RHKKRLLVVR ELKEQAQQAG LDDEVPHGQE SDLFSETSSV VSGSEMSGKY SHSNSRISAR SSKNRRKAER KKHSLKEGSP LEDLALLEAL SEVVQNTENL KDEVYHILKV LFLFEFDEQG RELQKAFEDT LQLMERSLPE IWTLTYQQNS ATPVLGPNST ANSIMASYQQ QKTSVPVLDA ELFIPPKINR RTQWKLSLLD; Positive Samples: HeLa, HepG2, U-251MG, HL-60, Mouse liver, Rat liver; Cellular Location: Cytoplasm, Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.94 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of IKAP in extracts of various cell lines. Samples were incubated with IKAP Polyclonal antibody (Product # PA5-87654) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of IKAP in paraffin-embedded rat stomach. Samples were incubated with IKAP Polyclonal antibody (Product # PA5-87654) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of IKAP in paraffin-embedded human liver cancer. Samples were incubated with IKAP Polyclonal antibody (Product # PA5-87654) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of IKAP in paraffin-embedded mouse kidney. Samples were incubated with IKAP Polyclonal antibody (Product # PA5-87654) using a dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.