Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA5-29112 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IKAP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, HepG2, GL261, Raw264.7. Predicted reactivity: Mouse (86%), Rat (85%), Rabbit (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IKAP Polyclonal Antibody (Product # PA5-29112). Sample (30 µg of whole cell lysate). Lane A: HeLa. Lane B: HepG2. 5% SDS PAGE. IKAP Polyclonal Antibody (Product # PA5-29112) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IKAP Polyclonal Antibody detects IKAP protein by western blot analysis. A. 30 µg GL261 whole cell lysate/extract. B. 30 µg Raw264.7 whole cell lysate/extract.5% SDS-PAGE. IKAP Polyclonal Antibody (Product # PA5-29112) dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IKAP Polyclonal Antibody detects IKAP protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: IKAP protein stained by IKBKAP antibody [C1C2], Internal (Product # PA5-29112) diluted at 1:500. Blue: Hoechst 33343 staining.