PA5-29112

antibody from Invitrogen Antibodies

Targeting: ELP1 DYS, IKAP, IKBKAP, IKI3, TOT1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-29112

Provider

Invitrogen Antibodies

Product name

IKAP Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HeLa, HepG2, GL261, Raw264.7.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using IKAP Polyclonal Antibody (Product # PA5-29112). Sample (30 µg of whole cell lysate). Lane A: HeLa. Lane B: HepG2. 5% SDS PAGE. IKAP Polyclonal Antibody (Product # PA5-29112) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IKAP Polyclonal Antibody detects IKAP protein by western blot analysis. A. 30 µg GL261 whole cell lysate/extract. B. 30 µg Raw264.7 whole cell lysate/extract.5% SDS-PAGE. IKAP Polyclonal Antibody (Product # PA5-29112) dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IKAP Polyclonal Antibody detects IKAP protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: IKAP protein stained by IKBKAP antibody [C1C2], Internal (Product # PA5-29112) diluted at 1:500. Blue: Hoechst 33343 staining.