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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Other assay [1]
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- Product number
- PA5-30634 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- JMJD1B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32. Predicted reactivity: Mouse (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of KDM3B using 30 µg of NT2D1 lysate. Samples were loaded onto a 5% SDS-PAGE gel and probed with a KDM3B polyclonal antibody (Product # PA5-30634) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using JMJD1B Polyclonal Antibody (Product # PA5-30634). NT2D1 whole cell and nuclear extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with JMJD1B Polyclonal Antibody (Product # PA5-30634) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- JMJD1B Polyclonal Antibody immunoprecipitates KDM3B protein in IP experiments. IP samples: NT2D1 whole cell extract. A. 50 µg NT2D1 whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of KDM3B protein by 4 µg JMJD1B Polyclonal Antibody (Product # PA5-30634). 5 % SDS-PAGE. The immunoprecipitated KDM3B protein was detected by JMJD1B Polyclonal Antibody (Product # PA5-30634) diluted at 1:500.
