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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunoprecipitation [2]
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- Product number
- NB100-81670 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-81670, RRID:AB_1146575
- Product name
- Rabbit Polyclonal PF1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PF1 Antibody [NB100-81670] - Detection of Human PF1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-PF1 antibody NB100-81670 used for IP at 3 mcg/mg lysate. PF1 was also immunoprecipitated by rabbit anti-PF1 antibody NB100-81671, which recognizes a downstream epitope. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PF1 Antibody [NB100-81670] - Detection of human PF1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-PF1 antibody NB100-81670 used for IP at 3 ug per reaction. PF1 was also immunoprecipitated by rabbit anti-PF1 antibody NB100-81671. For blotting immunoprecipitated PF1, NB100-81670 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
