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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-49447 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ITGA7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of ITGA7 in human cervical epithelial adenocarcinoma HeLa cells. Cells were fixed, permeabilized and stained with ITGA7 polyclonal antibody (Product # PA5-49447) at a 1:25 dilution, followed by Dylight® 488-conjugated goat rabbit IgG (NK179883) secondary antibody at 1:200 dilution (green). Cytoplasmic actin is detected with Dylight® 554 Phalloidin (PD18466410) at 1:100 dilution (red). The nuclear counter stain is DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of ITGA7 in U-2 OS cells. Cells were fixed, permeabilized, and incubated with ITGA7 polyclonal antibody (Product # PA5-49447, green line) at a 1:25 dilution. After incubation with the primary antibody for 1 hour at 37°C, cells were incubated with a Goat-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed antibody at 1/400 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.
