Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX632425 - Provider product page
- Provider
- GeneTex
- Product name
- Filamin B antibody [GT387]
- Antibody type
- Monoclonal
- Reactivity
- Human, Rat
- Host
- Mouse
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody [GT387] (GTX632425) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Filamin B antibody [GT387] detects Filamin B protein by western blot analysis. Various whole cell extracts (30 £gg) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody [GT387] (GTX632425) diluted at a dilution of 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) HeLa whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody [GT387] (GTX632425) diluted at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Filamin B antibody [GT387] detects Filamin B protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Filamin B protein stained by Filamin B antibody [GT387] (GTX632425) diluted at 1:200.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Filamin B antibody [GT387] detects Filamin B protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. Filamin B antibody [GT387] (GTX632425) diluted at 1:500.