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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [10]
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- Product number
- PA5-52098 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Filamin B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: KEPGEYAVHI MCDDEDIKDS PYMAFIHPAT GGYNPDLVRA YGPGLEKSGC IVNNLAEFTV DPKDAGKAPL KIFAQDGEGQ RIDIQMKNRM DGTYACSYTP VKAIKHTIAV VWG
- Concentration
- 0.2 mg/mL
Submitted references Proteome Analysis of Isolated Podocytes Reveals Stress Responses in Glomerular Sclerosis.
Koehler S, Kuczkowski A, Kuehne L, Jüngst C, Hoehne M, Grahammer F, Eddy S, Kretzler M, Beck BB, Höhfeld J, Schermer B, Benzing T, Brinkkoetter PT, Rinschen MM
Journal of the American Society of Nephrology : JASN 2020 Mar;31(3):544-559
Journal of the American Society of Nephrology : JASN 2020 Mar;31(3):544-559
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Filamin B in mouse cell line NIH-3T3 and rat cell line NBT-II using a Filamin B Polyclonal Antibody (Product # PA5-52098).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Filamin B in human cell lines A-549 and HEK293 using a Filamin B Polyclonal Antibody (Product # PA5-52098). Corresponding FLNB RNA-seq data are presented for the same cell lines. Loading control: Anti-HSP90B1.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Filamin B in mouse cell line NIH-3T3 and rat cell line NBT-II using a Filamin B Polyclonal Antibody (Product # PA5-52098).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent staining of Filamin B in human cell line U-2 OS shows positivity in plasma membrane & cytoplasm. Samples were probed using a Filamin B Polyclonal Antibody (Product # PA5-52098).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Filamin B in human testis using Filamin B Polyclonal Antibody (Product # PA5-52098).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Filamin B in human pancreas using Filamin B Polyclonal Antibody (Product # PA5-52098).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Filamin B in human kidney using Filamin B Polyclonal Antibody (Product # PA5-52098).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Filamin B in human colon using Filamin B Polyclonal Antibody (Product # PA5-52098).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Filamin B in human prostate and skeletal muscle tissues using Filamin B Polyclonal Antibody (Product # PA5-52098). Corresponding FLNB RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Filamin B in human colon, kidney, pancreas and testis using Filamin B Polyclonal Antibody (Product # PA5-52098) (A) shows similar protein distribution across tissues to an independent Filamin B Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Filamin B in human skin using Filamin B Polyclonal Antibody (Product # PA5-52098) shows weak to moderate cytoplasmic positivity in keratinocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Filamin B in human duodenum using Filamin B Polyclonal Antibody (Product # PA5-52098) shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Filamin B in human prostate using Filamin B Polyclonal Antibody (Product # PA5-52098) shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Filamin B in human skeletal muscle using Filamin B Polyclonal Antibody (Product # PA5-52098) shows weak to moderate cytoplasmic positivity in myocytes.
