Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunocytochemistry [2]
- Immunohistochemistry [6]
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- Product number
- PA5-52098 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Filamin B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Immunogen sequence: KEPGEYAVHI MCDDEDIKDS PYMAFIHPAT GGYNPDLVRA YGPGLEKSGC IVNNLAEFTV DPKDAGKAPL KIFAQDGEGQ RIDIQMKNRM DGTYACSYTP VKAIKHTIAV VWG Highest antigen sequence identity to the following orthologs: Mouse - 90%, Rat - 89%.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.2 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Proteome Analysis of Isolated Podocytes Reveals Stress Responses in Glomerular Sclerosis.
Koehler S, Kuczkowski A, Kuehne L, Jüngst C, Hoehne M, Grahammer F, Eddy S, Kretzler M, Beck BB, Höhfeld J, Schermer B, Benzing T, Brinkkoetter PT, Rinschen MM
Journal of the American Society of Nephrology : JASN 2020 Mar;31(3):544-559
Journal of the American Society of Nephrology : JASN 2020 Mar;31(3):544-559
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Immunofluorescent staining of Filamin B in human cell line U-2 OS shows positivity in plasma membrane & cytoplasm. Samples were probed using a Filamin B Polyclonal Antibody (Product # PA5-52098).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorecent analysis of Filamin B in human cell line U-2 OS using Filamin B Polyclonal Antibody (Product # PA5-52098). Staining shows localization to plasma membrane and cytosol.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Filamin B in human duodenum using Filamin B Polyclonal Antibody (Product # PA5-52098) shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Filamin B in human prostate using Filamin B Polyclonal Antibody (Product # PA5-52098) shows moderate to strong cytoplasmic positivity in glandular cells.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Filamin B in human prostate and skeletal muscle tissues using Filamin B Polyclonal Antibody (Product # PA5-52098). Corresponding FLNB RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Filamin B in human colon, kidney, pancreas and testis using Filamin B Polyclonal Antibody (Product # PA5-52098) (A) shows similar protein distribution across tissues to an independent Filamin B Polyclonal Antibody (B).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Filamin B in human skin using Filamin B Polyclonal Antibody (Product # PA5-52098) shows weak to moderate cytoplasmic positivity in keratinocytes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of Filamin B in human skeletal muscle using Filamin B Polyclonal Antibody (Product # PA5-52098) shows weak to moderate cytoplasmic positivity in myocytes.