Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- MA5-27748 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Filamin B Monoclonal Antibody (GT387)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: A431, HeLa, HepG2 Predicted Reactivity: Mouse (98%), Chicken (97%), Bovine (99%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- GT387
- Vial size
- 100 µL
- Concentration
- 1.64 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Filamin B in whole cell lysate using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with Filamin B monoclonal antibody (Product # MA5-27748) using a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Filamin B in non-transfected (-) and transfected (+) HeLa cells using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with Filamin B monoclonal antibody (Product # MA5-27748) using a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Filamin B Monoclonal Antibody (GT387) detects Filamin B protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B Monoclonal Antibody (GT387) (Product # MA5-27748) diluted at a dilution of 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Filamin B Monoclonal Antibody (GT387) detects Filamin B protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Filamin B protein stained by Filamin B Monoclonal Antibody (GT387) (Product # MA5-27748) diluted at 1:200. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Filamin B Monoclonal Antibody (GT387) detects Filamin B protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. Filamin B Monoclonal Antibody (GT387) (Product # MA5-27748) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.