MA5-27748

antibody from Invitrogen Antibodies

Targeting: FLNB ABP-278, FH1, FLN1L, LRS1, TABP, TAP

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-27748

Provider

Invitrogen Antibodies

Product name

Filamin B Monoclonal Antibody (GT387)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Control: A431, HeLa, HepG2 Predicted Reactivity: Mouse (98%), Chicken (97%), Bovine (99%) Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human, Rat

Host

Mouse

Isotype

IgG

Antibody clone number

GT387

Vial size

100 µL

Concentration

1.64 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Filamin B in whole cell lysate using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with Filamin B monoclonal antibody (Product # MA5-27748) using a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Filamin B in non-transfected (-) and transfected (+) HeLa cells using 30 µg of protein. Samples were separated with 5% SDS-PAGE and incubated with Filamin B monoclonal antibody (Product # MA5-27748) using a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Filamin B Monoclonal Antibody (GT387) detects Filamin B protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B Monoclonal Antibody (GT387) (Product # MA5-27748) diluted at a dilution of 1:1,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Filamin B Monoclonal Antibody (GT387) detects Filamin B protein at cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Filamin B protein stained by Filamin B Monoclonal Antibody (GT387) (Product # MA5-27748) diluted at 1:200. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Filamin B Monoclonal Antibody (GT387) detects Filamin B protein at cytoplasm in rat testis by immunohistochemical analysis. Sample: Paraffin-embedded rat testis. Filamin B Monoclonal Antibody (GT387) (Product # MA5-27748) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.