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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [3]
- Immunocytochemistry [3]
- Immunohistochemistry [1]
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- Product number
- PA5-21345 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Filamin B Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2, U87-MG, NIH-3T3. Predicted reactivity: Mouse (98%), Rat (98%), Chicken (97%), Bovine (99%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.296 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Filamin B Polyclonal Antibody (Product # PA5-21345). Sample (30 µg of whole cell lysate). Lane A: U87-MG. 5% SDS PAGE. Filamin B Polyclonal Antibody (Product # PA5-21345) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Filamin B antibody detects Filamin B protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with Filamin B antibody Filamin B Polyclonal Antibody (Product # PA5-21345) diluted at a dilution of 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Filamin B Polyclonal Antibody detects Filamin B protein by western blot analysis. A. 30 µg NIH-3T3 whole cell extract.5% SDS-PAGE. Filamin B Polyclonal Antibody (Product # PA5-21345) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Filamin B in paraformaldehyde-fixed HeLa cells using a Filamin B polyclonal antibody (Product # PA5-21345) (Green) at a 1:500 dilution. Alpha-tubulin filaments were labeled with Product # PA5-29281 (Red) at a 1:2500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Filamin B antibody (Product # PA5-21345) (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:2500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using Filamin B antibody (Product # PA5-21345) (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Product # MA1-25054) (Red) at 1:2500.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Filamin B was performed in paraffin-embedded human lung carcinoma tissue using Filamin B Polyclonal Antibody (Product # PA5-21345) at a dilution of 1:500.
