H00005495-D01P

antibody from Abnova Corporation

Targeting: PPM1B PP2CB, PP2CBETA, PPC2BETAX

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005495-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005495-D01P, RRID:AB_1578988

Product name

PPM1B purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human PPM1B protein.

Antigen sequence

MGAFLDKPKTEKHNAHGAGNGLRYGLSSMQGWRVE
MEDAHTAVVGIPHGLEDWSFFAVYDGHAGSRVANY
CSTHLLEHITTNEDFRAAGKSGSALELSVENVKNG
IRTGFLKIDEYMRNFSDLRNGMDRSGSTAVGVMIS
PKHIYFINCGDSRAVLYRNGQVCFSTQDHKPCNPR
EKERIQNAGGSVMIQRVNGSLAVSRALGDYDYKCV
DGKGPTEQLVSPEPEVYEILRAEEDEFIILACDGI
WDVMSNEELCEYVKSRLEVSDDLENVCNWVVDTCL
HKGSRDNMSIVLVCFSNAPKVSDEAVKKDSELDKH
LESRVEEIMEKSGEEGMPDLAHVMRILSAENIPNL
PPGGGLAGKRNVIEAVYSRLNPHRESDGASDEAEE
SGSQGKLVEALRQMRINHRGNYRQLLEEMLTSYRL
AKVEGEESPAEPAATATSSNSDAGNPVTMQESHTE
SESGLAELDSSNEDAGTKMSGEKI


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of PPM1B expression in transfected 293T cell line (H00005495-T01) by PPM1B MaxPab polyclonal antibody.Lane 1: PPM1B transfected lysate(52.60 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between PPM1B and CHUK. HeLa cells were stained with anti-PPM1B rabbit purified polyclonal 1:1200 and anti-CHUK mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)