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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
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Validation data
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- Product number
- AM03184PU-N - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#AM03184PU-N, RRID:AB_10422294
- Product name
- anti Protein phosphatase 1B / PPM1B
- Antibody type
- Monoclonal
- Antigen
- Recombinant human PPM1B (288-479aa) purified from E. coli.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- k1B1
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western blot analysis Cell lysates of MCF7(30 µg) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPM1B (172,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Cell lysates of MCF7(30ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human PPM1B (1:2,000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
