A02859-1

antibody from Boster Biological Technology

Targeting: TLN1 ILWEQ, TLN

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

A02859-1

Provider

Boster Biological Technology

Product name

Anti-Talin 1/TLN1 Antibody Picoband™

Antibody type

Polyclonal

Description

Rabbit IgG polyclonal antibody for Talin 1 detection. Tested with WB, IHC-P in Human;Mouse;Rat.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

References

Submitted references

Inward Tension of Talin and Integrin-related Osmotic Pressure are involved Synergetically in the Invasion and Metastasis of Non-small Cell Lung Cancer.

Song Y, Li C, Fu Y, Xie Q, Guo J, Li G, Wu H
Journal of Cancer 2020;11(17):5032-5041

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Talin promotes integrin activation accompanied by generation of tension in talin and an increase in osmotic pressure in neurite outgrowth.

Wang Y, Zhang X, Tian J, Shan J, Hu Y, Zhai Y, Guo J
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2019 May;33(5):6311-6326

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of Talin 1 using anti-Talin 1 antibody (A02859-1).Talin 1 was detected in paraffin-embedded section of human Lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-Talin 1 Antibody (A02859-1) overnight at 4℃. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37℃. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.

Additional image