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LearnA301-228A
antibody from Invitrogen Antibodies
Targeting: BRF1
BRF, GTF3B, hBRF, TAF3B2, TAF3C, TFIIIB90
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- A301-228A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- BRF1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.20 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse BRF1 by western blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 µg), HEK293T (T; 50 µg), and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-BRF1 antibody (Product # A301-228A; lot A301-228A-1) used for WB at 0.04 µg/mL. Detection: Chemiluminescence with exposure time of 10 seconds.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human and mouse BRF1 by western blot (h & m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 µg), and mouse NIH3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-BRF1 antibody A301-228A used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 3 µg/mg lysate. BRF1 was also immunoprecipitated by rabbit anti-BRF1 antibody A301-227A, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BRF1 by immunohistochemistry. Sample: FFPE section of human thyroid carcinoma. Antibody: Affinity purified rabbit anti-BRF1 (Cat. No. A301-228A) used at a dilution of 1:200 (1µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human BRF1 by western blot of immunoprecipitation. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BRF1 antibody (Product # A301-228A; lot A301-228A-1) used for IP at 3 µg/mg lysate. BRF1 was also immunoprecipitated by rabbit anti-BRF1 antibody (Product # A301-227A), which recognizes an upstream epitope. For blotting immunoprecipitated BRF1 (Product # A301-228A) was used at 1.0 µg/mL. Detection: Chemiluminescence with exposure time of 3 seconds.
