Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-22216 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CTNNAL1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2. Predicted reactivity: Mouse (89%), Rat (89%), Bovine (94%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CTNNAL1 Polyclonal Antibody (Product # PA5-22216). Sample (30 µg of whole cell lysate). Lane A: Hela. Lane B: Hep G2 . 7.5% SDS PAGE. CTNNAL1 Polyclonal Antibody (Product # PA5-22216) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Alpha-catulin antibody [N3C2], Internal detects Alpha-catulin protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: Alpha-catulin protein stained by Alpha-catulin antibody [N3C2], Internal (Product # PA5-22216) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Alpha-catulin antibody [N3C2], Internal detects Alpha-catulin protein at cytoplasm on mouse cervix by immunohistochemical analysis. Sample: Paraffin-embedded mouse cervix. Alpha-catulin antibody [N3C2], Internal (Product # PA5-22216) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft , using Alpha-catulin (Product # PA5-22216) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.