Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Immunohistochemistry [1]
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- Product number
- PA1680 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-PI 3 Kinase p85 beta/PIK3R2 Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for PI 3 KINASE P85 BETA/PIK3R2 detection. Host: Rabbit.Size: 100μg/vial. Tested applications: IHC-P. Reactive species: Human. PI 3 KINASE P85 BETA/PIK3R2 information: Molecular Weight: 81545 MW; .
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100μg/vial
- Concentration
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
- Handling
- Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Submitted references PI3K p85 β regulatory subunit deficiency does not affect NK cell differentiation and increases NKG2D-mediated activation.
Rojas JM, Spada R, Sanz-Ortega L, Morillas L, Mejías R, Mulens-Arias V, Pérez-Yagüe S, Barber DF
Journal of leukocyte biology 2016 Dec;100(6):1285-1296
Journal of leukocyte biology 2016 Dec;100(6):1285-1296
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Supportive validation
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- Boster Biological Technology (provider)
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- Experimental details
- IHC analysis of PIK3R2 using anti-PIK3R2 antibody (PA1680). PIK3R2 was detected in paraffin-embedded section of human mammary cancer tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-PIK3R2 Antibody (PA1680) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
- Additional image