Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- LS-B28 - Provider product page
- Provider
- LSBio
- Product name
- IHC-plus™ ZIF268 / EGR1 Antibody LS-B28
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at 4°C or -20°C. Avoid freeze-thaw cycles.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-EGR-1 Antibody - Western Blot. Western blot of Affinity Purified anti-EGR-1 antibody shows detection of a predominant band at ~58 kD corresponding to EGR-1 present in mouse embryonic fibroblast whole cell lysate (arrowhead). Approximately 35 ug of lysate was separated by 4-20% SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1500. Reaction occurred 2 hours at room temperature followed by washes and reaction with a 1:10000 dilution of IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX (for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.