F48309

antibody from NSJ Bioreagents

Targeting: EGR1 AT225, G0S30, KROX-24, NGFI-A, TIS8, ZIF-268, ZNF225

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F48309

Provider

NSJ Bioreagents

Product name

EGR1 Antibody

Antibody type

Polyclonal

Description

This highly specific EGR1 antibody is suitable for use in Western blot/Flow cytometry/Immunofluorescence/ELISA applications with human and mouse samples.

Reactivity

Human, Mouse

Host

Rabbit

Conjugate

Unconjugated

Vial size

0.08 ml, 0.4 ml

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the EGR1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

EGR1 antibody western blot analysis in MDA-MB231 lysate. Predicted molecular weight ~58 kDa.

Immunocytochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of EGR1 antibody with HeLa cells followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used as a nuclear counterstain (blue).

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

EGR1 antibody flow cytometric analysis of WiDr cells (right histogram) compared to a negative control (left histogram). FITC-conjugated goat-anti-rabbit secondary Ab was used for the analysis.