Explore
Validate
LearnNBP1-78775
antibody from Novus Biologicals
Targeting: EGR1
AT225, G0S30, KROX-24, NGFI-A, TIS8, ZIF-268, ZNF225
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-78775 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-78775, RRID:AB_11016873
- Product name
- Rabbit Polyclonal EGR1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Egr1 mediates the effect of insulin on leptin transcription in adipocytes.
Mohtar O, Ozdemir C, Roy D, Shantaram D, Emili A, Kandror KV
The Journal of biological chemistry 2019 Apr 12;294(15):5784-5789
The Journal of biological chemistry 2019 Apr 12;294(15):5784-5789
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: EGR1 Antibody [NBP1-78775] - Whole cell lysate from mouse bone marrow dendritic (D; 50 and 15 mcg for WB; 1 mg for IP, 20% of IP loaded), NIH3T3 (M; 50 mcg), human erythroleukemia (E; 50 mcg), and Jurkat (J; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-EGR1 antibody used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. EGR1 was also immunoprecipitated by rabbit anti-EGR1 antibody NBP1-78776 which recognizes a disparate epitope.
