MA5-15008

antibody from Invitrogen Antibodies

Targeting: EGR1 AT225, G0S30, KROX-24, NGFI-A, TIS8, ZIF-268, ZNF225

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Chromatin Immunoprecipitation (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15008

Provider

Invitrogen Antibodies

Product name

EGR1 Monoclonal Antibody (T.160.5)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

T.160.5

Vial size

100 µL

Concentration

42 µg/mL

Storage

-20°C

References

Submitted references

Identification and Validation of Potential Biomarkers and Their Functions in Acute Kidney Injury.

Chen J, Chen Y, Olivero A, Chen X
Frontiers in genetics 2020;11:411

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Fasting-mimicking diet and hormone therapy induce breast cancer regression.

Caffa I, Spagnolo V, Vernieri C, Valdemarin F, Becherini P, Wei M, Brandhorst S, Zucal C, Driehuis E, Ferrando L, Piacente F, Tagliafico A, Cilli M, Mastracci L, Vellone VG, Piazza S, Cremonini AL, Gradaschi R, Mantero C, Passalacqua M, Ballestrero A, Zoppoli G, Cea M, Arrighi A, Odetti P, Monacelli F, Salvadori G, Cortellino S, Clevers H, De Braud F, Sukkar SG, Provenzani A, Longo VD, Nencioni A
Nature 2020 Jul;583(7817):620-624

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METTL3-mediated N(6)-methyladenosine mRNA modification enhances long-term memory consolidation.

Zhang Z, Wang M, Xie D, Huang Z, Zhang L, Yang Y, Ma D, Li W, Zhou Q, Yang YG, Wang XJ
Cell research 2018 Nov;28(11):1050-1061

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Melatonin attenuates scopolamine-induced memory/synaptic disorder by rescuing EPACs/miR-124/Egr1 pathway.

Wang X, Wang ZH, Wu YY, Tang H, Tan L, Wang X, Gao XY, Xiong YS, Liu D, Wang JZ, Zhu LQ
Molecular neurobiology 2013 Feb;47(1):373-81

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of EGR1 in dissociated PC12 cells using an EGR1 monoclonal antibody (Product # MA5-15008) (green) and a MEK1/2 monoclonal antibody (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 5 m 6 A promotes the translation of immediate-early genes upon activity induction. a Immediate-early genes (IEGs) are comparably induced by fear conditioning training in the hippocampus of both the CTRL (blue) and cKO (red) mice. b Increment of m 6 A modification abundance on IEG mRNAs after fear conditioning training in hippocampus samples of the CTRL mice but not the Mettl3 cKO mice. IP, immunoprecipitated fraction. c Translation of IEGs is impaired in the cKO mice after training. d Immunofluorescent image of EGR1 in CA1 region before and after fear conditioning training (1 h and 4 h). e Translation of IEGs is impaired in Mettl3 -KO primary cortical neurons, which can be rescued by re-expression of wildtype Mettl3 but not mutated Mettl3 . Student's t -test, * P < 0.05, ** P < 0.01, *** P < 0.001, N.S., not significant, ( a ) n = 2 replicates, ( b ), ( c ) and ( e ) n = 3 replicates

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 8 Expression of 35 key genes and EGR1 in AKI samples and sham samples. (A) Transcriptional levels of 35 important genes including 21 hub genes and 14 other important upregulated genes in our own AKI samples. (B) Translational levels of Egr1 in AKI samples and sham samples. (C) Western blot of EGR1. (D,E) Immunofluorescence of EGR1. * p < 0.05, ** p < 0.01, *** p < 0.001.