sc-46676

ITGAX antibody from Santa Cruz Biotechnology

CD11C

Western blot

Supportive data in Antibodypedia

Immunohistochemistry

Supportive data in Antibodypedia

Provider product page for sc-46676

Antibody Data

Product number

sc-46676

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-46676, RRID:AB_626859

Product name

Anti-ITGAX

Provider product page

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

Single band corresponding to the predicted size in kDa (+/-20%).

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 229, 112, 83.5, 47.9, 32.3, 26.5, 17.2

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 39

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human spleen shows strong cytoplasmic positivity in a subset of cells in red pulp.

Validation comment

Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.