AP6986b

antibody from Abcepta

Targeting: ITGAX CD11C

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP6986b

Provider

Abcepta

Proper citation

Abgent Cat#AP6986b, RRID:AB_1967389

Product name

ITGAX Antibody (C-term)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Western blot analysis of ITGAX Antibody (C-term) (Cat. #AP6986b) in MDA-MB231 cell line lysates (35ug/lane). ITGAX (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Immunofluorescence analysis of ITGAX Antibody (C-term) with paraffin-embedded human hepatocarcinoma . 0.05 mg/ml primary antibody was followed by FITC-conjugated goat anti-rabbit lgG (whole molecule). FITC emits green fluorescence.Red counterstaining is PI.

Primary Ab dilution

1:10~50

Immunohistochemistry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

"Formalin-fixed and paraffin-embedded human hepatocarcinoma with ITGAX Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated."

Primary Ab dilution

1:50~100

Flow cytometry

Supportive validation

Submitted by

Abcepta (provider)

Main image

Experimental details

Flow cytometric analysis of SK-Br-3 cells using ITGAX Antibody (C-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Primary Ab dilution

1:10~50