Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 47-0128-41 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-CD11c Monoclonal Antibody (BU15), APC-eFluor 780, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: The BU15 monoclonal antibody reacts with canine, human, and non-human primate CD11c, the 150 kDa integrin aX chain. CD11c non-covalently associates with beta2 integrin to form the CD11c/CD18 heterodimer. This complex is expressed on monocytes, granulocytes, macrophages, NK, dendritic cells, and subset of T and B lymphocytes. CD11c/CD18 binds to CD54, iC3b and fibrinogen and plays a role in leukocyte adhesive interactions. CD11c also binds complement fragment iC3b to mediate phagocytosis of opsonized particles. The BU15 antibody has been reported to crossreact to cynomologus monkey. Moreover, this antibody has been reported to exhibit blocking activity. Crossblocking studies suggest that BU15 recognizes a different epitope from clone 3.9. Applications Reported: This BU15 antibody has been reported for use in flow cytometric analysis. Applications Tested: This BU15 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome. Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically. Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser. Filtration: 0.2 µm post-manufacturing filtered.
- Reactivity
- Human, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- BU15
- Vial size
- 25 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Ozone enhances pulmonary innate immune response to a Toll-like receptor-2 agonist.
Sirtuin 1 promotes Th2 responses and airway allergy by repressing peroxisome proliferator-activated receptor-γ activity in dendritic cells.
Chronic helminth infection promotes immune regulation in vivo through dominance of CD11cloCD103- dendritic cells.
Oakes JL, O'Connor BP, Warg LA, Burton R, Hock A, Loader J, Laflamme D, Jing J, Hui L, Schwartz DA, Yang IV
American journal of respiratory cell and molecular biology 2013 Jan;48(1):27-34
American journal of respiratory cell and molecular biology 2013 Jan;48(1):27-34
Sirtuin 1 promotes Th2 responses and airway allergy by repressing peroxisome proliferator-activated receptor-γ activity in dendritic cells.
Legutko A, Marichal T, Fiévez L, Bedoret D, Mayer A, de Vries H, Klotz L, Drion PV, Heirman C, Cataldo D, Louis R, Thielemans K, Andris F, Leo O, Lekeux P, Desmet CJ, Bureau F
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 1;187(9):4517-29
Journal of immunology (Baltimore, Md. : 1950) 2011 Nov 1;187(9):4517-29
Chronic helminth infection promotes immune regulation in vivo through dominance of CD11cloCD103- dendritic cells.
Smith KA, Hochweller K, Hämmerling GJ, Boon L, MacDonald AS, Maizels RM
Journal of immunology (Baltimore, Md. : 1950) 2011 Jun 15;186(12):7098-109
Journal of immunology (Baltimore, Md. : 1950) 2011 Jun 15;186(12):7098-109
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Staining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (blue histogram) or Anti-CD11c APC-eFluor® 780 (purple histogram). Cells in the monocyte gate were used for analysis.