PA5-87590

antibody from Invitrogen Antibodies

Targeting: RPL8 L8

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-87590

Provider

Invitrogen Antibodies

Product name

RPL8 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MGRVIRGQRK GAGSVFRAHV KHRKGAARLR AVDFAERHGY IKGIVKDIIH DPGRGAPLAK VVFRDPYRFK KRTELFIAAE GIHTGQFVYC GKKAQLNIGN VLPVGTMPEG TIVCCLEEKP GDRGKLARAS GNYATVISHN PETKKTRVKL PSGSKKVISS ANRAVVGVVA GGGRIDKPIL KAGRAYHKYK AKRNCWPRVR GVAMNPVEHP FGGGNHQHIG KPSTIRRDAP AGRKVGLIAA RRTGRLRGTK TVQEKEN; Positive Samples: SKOV3, 293T, A-375, HepG2, MCF7, Mouse lung, Rat liver, Rat kidney; Cellular Location: Cytoplasm

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1.65 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of RPL8 was performed in HeLa cells using RPL8 Polyclonal Antibody (Product # PA5-87590) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of RPL8 was performed in HeLa cells using RPL8 Polyclonal Antibody (Product # PA5-87590) at a dilution of 1:200. Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of RPL8 in HeLa cells. Samples were incubated with RPL8 Polyclonal antibody (Product # PA5-87590) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Confocal immunofluorescence (Immunocytochemistry) analysis of RPL8 in HeLa cells. Samples were incubated with RPL8 Polyclonal antibody (Product # PA5-87590) using a dilution of 1:200. Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPL8 in paraffin-embedded rat brain. Samples were incubated with RPL8 Polyclonal antibody (Product # PA5-87590) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPL8 in paraffin-embedded mouse brain. Samples were incubated with RPL8 Polyclonal antibody (Product # PA5-87590) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of RPL8 in paraffin-embedded mouse heart. Samples were incubated with RPL8 Polyclonal antibody (Product # PA5-87590) using a dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.