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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- GTX104073 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX104073, RRID:AB_1950542
- Product name
- IDH3A antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references β-Cell deletion of Nr4a1 and Nr4a3 nuclear receptors impedes mitochondrial respiration and insulin secretion.
Reynolds MS, Hancock CR, Ray JD, Kener KB, Draney C, Garland K, Hardman J, Bikman BT, Tessem JS
American journal of physiology. Endocrinology and metabolism 2016 Jul 1;311(1):E186-201
American journal of physiology. Endocrinology and metabolism 2016 Jul 1;311(1):E186-201
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sample (20 ?g of whole cell lysate) A: mouse brain 10% SDS PAGE GTX104073 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IDH3A antibody detects IDH3A protein by western blot analysis.A. 50 ?g rat brain lysate/extract10% SDS-PAGEIDH3A antibody (GTX104073) dilution: 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample(30 ?g whole cell lysate)A: HepG2 (GTX27900) 12% SDS PAGEGTX104073 diluted at 1:500The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- IDH3A antibody detects IDH3A protein at Mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37¢J for 30 min.Green: IDH3A protein stained by IDH3A antibody (GTX104073) diluted at 1:500.Blue: Hoechst 33343 staining.
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- GeneTex (provider)
- Main image
- Experimental details
- IDH3A antibody detects IDH3A protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: IDH3A protein stained by IDH3A antibody (GTX104073) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung SCC, using IDH3A(GTX104073) antibody at 1:100 dilution.
