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Western blot
ELISA
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunoprecipitation [2]
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Validation data
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- Product number
- LS-C670518 - Provider product page
- Provider
- LSBio
- Product name
- IDH3A Antibody LS-C670518
- Antibody type
- Polyclonal
- Description
- Protein G purified
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Enhanced validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Positive Western Blot detected in HepG2 whole cell lysat, Jurkat whole cell lysate, Rat brain tissue, Mouse brain tissue, Mouse liver tissue. All lanes: IDH3A antibody at 3.4 µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 40, 32 KDa. Observed band size: 40 KDa
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Immunoprecipitating IDH3A in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of IDH3A Antibody in HepG2 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: IDH3A Antibody (6µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (20µg)
- Submitted by
- LSBio (provider)
- Main image
- Experimental details
- Immunoprecipitating IDH3A in HepG2 whole cell lysate Lane 1: Rabbit control IgG instead of IDH3A Antibody in HepG2 whole cell lysate.For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000) Lane 2: IDH3A Antibody (6µg) + HepG2 whole cell lysate (500µg) Lane 3: HepG2 whole cell lysate (20µg)
