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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-97851 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IDH3A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 4.76 mg/mL
- Storage
- -20°C or -80°C if preferred
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of precipitated IDH3A from HepG2 whole cell lysate using a IDH3A polyclonal antibody (Product # PA5-97851). An HRP-conjugated Protein G antibody was used as the secondary antibody (1:2000). Lane 1: Rabbit control IgG. Lane 2: HepG2 whole cell lysate (500µg). Lane 3: HepG2 whole cell lysate (20µg).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of precipitated IDH3A from HepG2 whole cell lysate using a IDH3A polyclonal antibody (Product # PA5-97851). An HRP-conjugated Protein G antibody was used as the secondary antibody (1:2000). Lane 1: Rabbit control IgG. Lane 2: HepG2 whole cell lysate (500µg). Lane 3: HepG2 whole cell lysate (20µg).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of IDH3A using a IDH3A Polyclonal antibody (Product # PA5-97851) at a concentration of 3.4 µg/mL. Positive WB detected in: HepG2 whole cell lysate, Jurkat whole cell lysate, Rat brain tissue, Mouse brain tissue, Mouse liver tissue. A secondary Goat polyclonal antibody to rabbit IgG was applied at a 1:50,000 dilution. Observed band size: 40 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of IDH3A in A549 cells using a IDH3A polyclonal antibody (Product # PA5-97851) at a dilution of 1:100. Alexa Fluor 488-congugated Goat Anti-Rabbit IgG(H+L) secondary antibody was used.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of IDH3A in paraffin embedded human adrenal gland tissue using a IDH3A polyclonal antibody (Product # PA5-97851) at a dilution of 1:100.
