PA5-27992

antibody from Invitrogen Antibodies

Targeting: IDH3A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27992

Provider

Invitrogen Antibodies

Product name

IDH3A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2, mouse brain, rat brain.

Concentration

0.84 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using IDH3A Polyclonal Antibody (Product # PA5-27992). Sample (30 µg whole cell lysate). A: HepG2. 12% SDS PAGE. IDH3A Polyclonal Antibody (Product # PA5-27992) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IDH3A Polyclonal Antibody detects IDH3A protein by western blot analysis. A. 50 µg rat brain lysate/extract.10% SDS-PAGE. IDH3A Polyclonal Antibody (Product # PA5-27992) dilution: 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using IDH3A Polyclonal Antibody (Product # PA5-27992). Sample (20 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. IDH3A Polyclonal Antibody (Product # PA5-27992) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of IDH3A was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: IDH3A Polyclonal Antibody (Product # PA5-27992) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human lung SCC, using IDH3A (Product # PA5-27992) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.