Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PA5-27992 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IDH3A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, mouse brain, rat brain. Predicted reactivity: Mouse (98%), Rat (98%), Zebrafish (91%), Xenopus laevis (92%), Chicken (94%), Rhesus Monkey (99%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.84 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IDH3A Polyclonal Antibody (Product # PA5-27992). Sample (30 µg whole cell lysate). A: HepG2. 12% SDS PAGE. IDH3A Polyclonal Antibody (Product # PA5-27992) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IDH3A Polyclonal Antibody detects IDH3A protein by western blot analysis. A. 50 µg rat brain lysate/extract.10% SDS-PAGE. IDH3A Polyclonal Antibody (Product # PA5-27992) dilution: 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using IDH3A Polyclonal Antibody (Product # PA5-27992). Sample (20 µg of whole cell lysate). Lane A: mouse brain. 10% SDS PAGE. IDH3A Polyclonal Antibody (Product # PA5-27992) diluted at 1:10,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of IDH3A was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: IDH3A Polyclonal Antibody (Product # PA5-27992) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human lung SCC, using IDH3A (Product # PA5-27992) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.