HPA063876

antibody from Atlas Antibodies

Targeting: GOLGA5 golgin-84, GOLIM5, ret-II, rfg5

Antibody data

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Validation data

Product number: HPA063876 - Provider product page
Provider: Atlas Antibodies
Proper citation: Atlas Antibodies Cat#HPA063876, RRID:AB_2685140
Product name: Anti-GOLGA5
Antibody type: Polyclonal
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Antigen sequence: QEFHYIEEDLYRTKNTLQSRIKDRDEEIQKLRNQL TNKTLSNSSQSELENRLHQLTETLIQKQTMLESLS TEKNSLVFQLERLEQQMNS
Isotype: IgG
Vial size: 100 µl
Storage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

GOLGA5 protein structure - HPA063876 shown in red.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Orthogonal validation
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Experimental details: Immunohistochemistry analysis in human epididymis and skeletal muscle tissues using Anti-GOLGA5 antibody. Corresponding GOLGA5 RNA-seq data are presented for the same tissues.
Sample type: HUMAN
Orthogonal method: Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

Enhanced validation

Submitted by: Atlas Antibodies (provider)
Enhanced method: Independent antibody validation
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Experimental details: Immunohistochemical staining of human cerebral cortex, kidney, liver and testis using Anti-GOLGA5 antibody HPA063876 (A) shows similar protein distribution across tissues to independent antibody HPA000992 (B).
Antibody #2 product nr: HPA000992
Antibody provider: Atlas Antibodies

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human testis shows moderate, granular cytoplasmic and nuclear positivity in cells in seminiferous ducts and Leydig cells.

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human epididymis shows high expression.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human skeletal muscle shows low expression as expected.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human cerebral cortex using Anti-GOLGA5 antibody HPA063876.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human liver using Anti-GOLGA5 antibody HPA063876.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human testis using Anti-GOLGA5 antibody HPA063876.
Sample type: HUMAN

Supportive validation

Submitted by: Atlas Antibodies (provider)
Main image
Experimental details: Immunohistochemical staining of human kidney using Anti-GOLGA5 antibody HPA063876.
Sample type: HUMAN