MAB3225

antibody from Abnova Corporation

Targeting: ITGB2 CD18, LFA-1, MAC-1, MFI7

Western blot (Recommended by provider)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB3225

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#MAB3225, RRID:AB_10552650

Product name

Itgb2 monoclonal antibody, clone C71/16

Antibody type

Monoclonal

Antigen

Native Itgb2 from cell membrane of mouse T cell lymphoma BW5147.

Reactivity

Mouse

Host

Rat

Isotype

IgG

Vial size

500 µg

Storage

Store at 4°C.

References

Submitted references

Ins and outs of LFA-1.

Lub M, van Kooyk Y, Figdor CG
Immunology today 1995 Oct;16(10):479-83

---

Molecular complexity of leukocyte surface glycoproteins related to the macrophage differentiation antigen Mac-1.

Trowbridge IS, Omary MB
The Journal of experimental medicine 1981 Nov 1;154(5):1517-24

---

Monoclonal xenogeneic antibodies to murine cell surface antigens: identification of novel leukocyte differentiation antigens.

Springer T, Galfrè G, Secher DS, Milstein C
European journal of immunology 1978 Aug;8(8):539-51

Flow cytometry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

BALB/c splenocytes were stained with Itgb2 monoclonal antibody, clone C71/16 (Cat # MAB3225). Small lymphocytes were then gated and analyzed on a FACScan™ flow cytometer (BDIS, San Jose, CA).

Validation comment

Flow Cytometry