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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [1]
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- Product number
- MAB6945 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#MAB6945, RRID:AB_10680303
- Product name
- ITGB2 monoclonal antibody, clone IB4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against native ITGB2.
- Isotype
- IgG
- Antibody clone number
- IB4
- Storage
- Store at 4°C.
Submitted references A crucial role for beta 2 integrin in the activation of eosinophils stimulated by IgG.
Leukocyte CD11/CD18 integrins: biological and clinical relevance.
Involvement of the "I" domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3.
Kaneko M, Horie S, Kato M, Gleich GJ, Kita H
Journal of immunology (Baltimore, Md. : 1950) 1995 Sep 1;155(5):2631-41
Journal of immunology (Baltimore, Md. : 1950) 1995 Sep 1;155(5):2631-41
Leukocyte CD11/CD18 integrins: biological and clinical relevance.
Mazzone A, Ricevuti G
Haematologica 1995 Mar-Apr;80(2):161-75
Haematologica 1995 Mar-Apr;80(2):161-75
Involvement of the "I" domain of LFA-1 in selective binding to ligands ICAM-1 and ICAM-3.
Landis RC, McDowall A, Holness CL, Littler AJ, Simmons DL, Hogg N
The Journal of cell biology 1994 Jul;126(2):529-37
The Journal of cell biology 1994 Jul;126(2):529-37
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- Abnova Corporation (provider)
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- Experimental details
- 5 X 105 cultured human HPB-MLT cells per tube were incubated 45 minutes on ice with 80 uL of ITGB2 monoclonal antibody, clone IB4 (Cat # MAB6945) at a concentration of 5 ug/mL. Cells were washed twice and incubated with Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed using a BD FACS Calibur. Cells stained positive (67% net) with a mean shift of 0.92 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration.
- Validation comment
- Flow Cytometry
