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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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Validation data
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- Product number
- BMS103BT - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD18 (LFA-1 beta) Monoclonal Antibody (R3.3), Biotin, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Description: BMS103 is also suitable as primary antibody in staining for FACS analysis, BMS103FI can be used for direct staining of cells, BMS103BT can be used in a 2-step procedure using the biotin-(strept)avidin system. BMS103 can be used as a neutralizing agent. Recommended CD18 is a 90-95 kDa type I transmembrane protein that heterodimerizes with the a chain of CD11a, CD11b and CD11c. The CD18/CD11a complex is also known as lymphocyte function-associated antigen-1 (LFA-1) and plays an important role in cellular adhesion and inflammatory reactions. CD18 is expressed broadly by human lymphocytes and monocytes. Peripheral blood granulocytes also express CD18, but at lower levels. Applications Tested: Flow Cytometry. Purity: >95%.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Biotin
- Isotype
- IgG
- Antibody clone number
- R3.3
- Vial size
- 100 Tests
- Concentration
- 10 µL/Test
- Storage
- 2-8°C
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Supportive validation
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- Invitrogen Antibodies (provider)
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- Experimental details
- Staining of human peripheral blood mononuclear cells with CD45 Pacific Blue, CD3 FITC and CD18 Biotin followed by APC conjugated streptavidin. As expected based on known relative expression patterns, CD18 clone R3.3 stains a subset of lymphocytes (pink), monocytes (orange) and granulocytes (blue).
- Conjugate
- Biotin
