MA1807

antibody from Invitrogen Antibodies

Targeting: ITGB2 CD18, LFA-1, MAC-1, MFI7

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1807

Provider

Invitrogen Antibodies

Product name

CD18 Monoclonal Antibody (TA-4)

Antibody type

Monoclonal

Antigen

Other

Description

MA1807 targets CD18 in FACS applications and shows reactivity with Rat samples.

Antibody clone number

TA-4

Concentration

1.0 mg/mL

References

Submitted references

VCAM-1 has a tissue-specific role in mediating interleukin-4-induced eosinophil accumulation in rat models: evidence for a dissociation between endothelial-cell VCAM-1 expression and a functional role in eosinophil migration.

Larbi KY, Allen AR, Tam FW, Haskard DO, Lobb RR, Silva PM, Nourshargh S
Blood 2000 Nov 15;96(10):3601-9

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Intratracheal injection of endotoxin and cytokines. IX. Contribution of CD11a/ICAM-1 to neutrophil emigration.

Tang WW, Yi ES, Remick DG, Wittwer A, Yin S, Qi M, Ulich TR
The American journal of physiology 1995 Nov;269(5 Pt 1):L653-9

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of CD18 Monoclonal Antibody (TA-4) was performed using 70% confluent log phase Jurkat cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD18 (TA-4) Mouse Monoclonal Antibody (Product # MA1807) at 2 µg/mL in 0.1% BSA, incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing membranous localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.