M00458-1

antibody from Boster Biological Technology

Targeting: ITGB2 CD18, LFA-1, MAC-1, MFI7

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

M00458-1

Provider

Boster Biological Technology

Product name

Anti-CD18 ITGB2 Antibody Picoband™ (monoclonal, 1A3/2A10)

Antibody type

Monoclonal

Description

Mouse IgG monoclonal antibody for CD18 detection. Tested with WB, IHC-P in Human.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1A3/2A10

Vial size

100μg/vial

Concentration

0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

Storage

At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.

Handling

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Immunohistochemistry

Supportive validation

Submitted by

Boster Biological Technology (provider)

Main image

Experimental details

IHC analysis of CD18 using anti-CD18 antibody (M00458-1). CD18 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CD18 Antibody (M00458-1) overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.

Additional image