Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [10]
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Validation data
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- Product number
- NBP1-88127 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-88127, RRID:AB_11005054
- Product name
- Rabbit Polyclonal Integrin beta 2/CD18 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Integrin beta 2/CD18 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Integrin beta 2/CD18 Antibody [NBP1-88127] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Integrin beta 2/CD18 Antibody [NBP1-88127] - Analysis using Anti-ITGB2 antibody NBP1-88127 (A) shows similar pattern to independent antibody NBP1-88128 (B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88127] - Staining of human spleen shows strong cytoplasmic positivity in cells in red pulp.
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- Novus Biologicals (provider)
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- Experimental details
- Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88127] - Staining of human skeletal muscle shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88127] - Staining of human lymph node.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88127] - Staining of human bone marrow.
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88127] - Analysis in human lymph node and skeletal muscle tissues. Corresponding Integrin beta 2/CD18 RNA-seq data are presented for the same tissues.
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88127] - Staining of human colon shows strong membranous positivity in lymphoid cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88127] - Staining of human gastrointestinal, lymph node, skeletal muscle and tonsil using Anti-Integrin beta 2/CD18 antibody NBP1-88127 (A) shows similar protein distribution across tissues to independent antibody NBP1-88128 (B).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88127] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88127] - Staining of human lymph node shows strong membranous positivity in germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Integrin beta 2/CD18 Antibody [NBP1-88127] - Staining of human tonsil shows strong membranous positivity in germinal center cells.