MAB336-100

antibody from R&D Systems

Targeting: CCL22 A-152E5.1, ABCD-1, DC/B-CK, MDC, MGC34554, SCYA22, STCP-1

Western blot (Data presented on provider website)

ELISA (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB336-100

Provider

R&D Systems

Product name

Human CCL22/MDC Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects human CCL22/MDC in ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant mouse CCL17, recombinant human (rh) CCL17, or rhCCL21 is observed.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

O00626.1


Isotype

IgG

Antibody clone number

57226

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

CCL22 is a biomarker of cartilage injury and plays a functional role in chondrocyte apoptosis.

Ren G, Whittaker JL, Leonard C, De Rantere D, Pang DSJ, Salo P, Fritzler M, Kapoor M, de Koning APJ, Jaremko JL, Emery CA, Krawetz RJ
Cytokine 2019 Mar;115:32-44

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Distinct roles for CCR4 and CXCR3 in the recruitment and positioning of regulatory T cells in the inflamed human liver.

Oo YH, Weston CJ, Lalor PF, Curbishley SM, Withers DR, Reynolds GM, Shetty S, Harki J, Shaw JC, Eksteen B, Hubscher SG, Walker LS, Adams DH
Journal of immunology (Baltimore, Md. : 1950) 2010 Mar 15;184(6):2886-98

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CCL17 and CCL22 chemokines within tumor microenvironment are related to accumulation of Foxp3+ regulatory T cells in gastric cancer.

Mizukami Y, Kono K, Kawaguchi Y, Akaike H, Kamimura K, Sugai H, Fujii H
International journal of cancer 2008 May 15;122(10):2286-93

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Specific recruitment of regulatory T cells into the CSF in lymphomatous and carcinomatous meningitis.

Haas J, Schopp L, Storch-Hagenlocher B, Fritzsching B, Jacobi C, Milkova L, Fritz B, Schwarz A, Suri-Payer E, Hensel M, Wildemann B
Blood 2008 Jan 15;111(2):761-6

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Generation of Th1 and Th2 chemokines by human eosinophils: evidence for a critical role of TNF-alpha.

Liu LY, Bates ME, Jarjour NN, Busse WW, Bertics PJ, Kelly EA
Journal of immunology (Baltimore, Md. : 1950) 2007 Oct 1;179(7):4840-8

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Specific recruitment of regulatory T cells in ovarian carcinoma fosters immune privilege and predicts reduced survival.

Curiel TJ, Coukos G, Zou L, Alvarez X, Cheng P, Mottram P, Evdemon-Hogan M, Conejo-Garcia JR, Zhang L, Burow M, Zhu Y, Wei S, Kryczek I, Daniel B, Gordon A, Myers L, Lackner A, Disis ML, Knutson KL, Chen L, Zou W
Nature medicine 2004 Sep;10(9):942-9

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Enhanced generation of helper T type 1 and 2 chemokines in allergen-induced asthma.

Liu L, Jarjour NN, Busse WW, Kelly EA
American journal of respiratory and critical care medicine 2004 May 15;169(10):1118-24

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CCR4 blockade does not inhibit allergic airways inflammation.

Conroy DM, Jopling LA, Lloyd CM, Hodge MR, Andrew DP, Williams TJ, Pease JE, Sabroe I
Journal of leukocyte biology 2003 Oct;74(4):558-63

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Chemotaxis Induced by CCL22/MDC and Neutralization by Human CCL22/MDC Antibody. Recombinant Human CCL22/MDC (Catalog # 336-MD) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL22/MDC (10 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL22/MDC Monoclonal Antibody (Catalog # MAB336). The ND₅₀ is typically 0.6-3.0 µg/mL.