Explore
Validate
LearnAF439
antibody from R&D Systems
Targeting: CCL22
A-152E5.1, ABCD-1, DC/B-CK, MDC, MGC34554, SCYA22, STCP-1
Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [8]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF439 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse CCL22/MDC Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse CCL22/MDC in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human CCL22/MDC is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9QZU2- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Eosinophil recruitment is dynamically regulated by interplay among lung dendritic cell subsets after allergen challenge.
IL-4/CCL22/CCR4 axis controls regulatory T-cell migration that suppresses inflammatory bone loss in murine experimental periodontitis.
Defective immunoregulation in RSV vaccine-augmented viral lung disease restored by selective chemoattraction of regulatory T cells.
Prevention of murine autoimmune diabetes by CCL22-mediated Treg recruitment to the pancreatic islets.
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells.
The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
Immunosuppressive effects of CCL17 on pulmonary antifungal responses during pulmonary invasive aspergillosis.
Yi S, Zhai J, Niu R, Zhu G, Wang M, Liu J, Huang H, Wang Y, Jing X, Kang L, Song W, Shi Y, Tang H
Nature communications 2018 Sep 24;9(1):3879
Nature communications 2018 Sep 24;9(1):3879
IL-4/CCL22/CCR4 axis controls regulatory T-cell migration that suppresses inflammatory bone loss in murine experimental periodontitis.
Araujo-Pires AC, Vieira AE, Francisconi CF, Biguetti CC, Glowacki A, Yoshizawa S, Campanelli AP, Trombone AP, Sfeir CS, Little SR, Garlet GP
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2015 Mar;30(3):412-22
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 2015 Mar;30(3):412-22
Defective immunoregulation in RSV vaccine-augmented viral lung disease restored by selective chemoattraction of regulatory T cells.
Loebbermann J, Durant L, Thornton H, Johansson C, Openshaw PJ
Proceedings of the National Academy of Sciences of the United States of America 2013 Feb 19;110(8):2987-92
Proceedings of the National Academy of Sciences of the United States of America 2013 Feb 19;110(8):2987-92
Prevention of murine autoimmune diabetes by CCL22-mediated Treg recruitment to the pancreatic islets.
Montane J, Bischoff L, Soukhatcheva G, Dai DL, Hardenberg G, Levings MK, Orban PC, Kieffer TJ, Tan R, Verchere CB
The Journal of clinical investigation 2011 Aug;121(8):3024-8
The Journal of clinical investigation 2011 Aug;121(8):3024-8
Comprehensive assessment of chemokine expression profiles by flow cytometry.
Eberlein J, Nguyen TT, Victorino F, Golden-Mason L, Rosen HR, Homann D
The Journal of clinical investigation 2010 Mar;120(3):907-23
The Journal of clinical investigation 2010 Mar;120(3):907-23
Allergic pulmonary inflammation in mice is dependent on eosinophil-induced recruitment of effector T cells.
Jacobsen EA, Ochkur SI, Pero RS, Taranova AG, Protheroe CA, Colbert DC, Lee NA, Lee JJ
The Journal of experimental medicine 2008 Mar 17;205(3):699-710
The Journal of experimental medicine 2008 Mar 17;205(3):699-710
The direct action of 1alpha,25(OH)2-vitamin D3 on purified mouse Langerhans cells.
Fujita H, Asahina A, Komine M, Tamaki K
Cellular immunology 2007 Feb;245(2):70-9
Cellular immunology 2007 Feb;245(2):70-9
Immunosuppressive effects of CCL17 on pulmonary antifungal responses during pulmonary invasive aspergillosis.
Carpenter KJ, Hogaboam CM
Infection and immunity 2005 Nov;73(11):7198-207
Infection and immunity 2005 Nov;73(11):7198-207
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Chemotaxis Induced by CCL22/MDC and Neutralization by Mouse CCL22/MDC Antibody. Recombinant Mouse CCL22/MDC (Catalog # 439-MD) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin. Chemotaxis elicited by Recombinant Mouse CCL22/MDC (15 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse CCL22/MDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF439). The ND50 is typically 0.3-1.0 µg/mL.
